M. Gautam et al., AMYLOPLASTS AND STARCH CHARACTERIZATION IN HIGH LYSINE BARLEY MUTANT, Journal of Plant Biochemistry and Biotechnology, 3(2), 1994, pp. 131-136
Starch granules of Notch-2 high lysine barley mutant are small, irregu
lar in shape and show a virtual loss of birefringence and a higher gel
atinization temperature, compared to parent NP-113. Starch polymer of
Notch-2 is qualitatively different from parent, as evidenced by X-ray
diffractograms. There is a higher incidence of V-pattern at early deve
lopment as also at maturity in mutant as compared to parent. Intensiti
es corresponding to B-pattern are present at early development in both
parent and mutant. B-pattern persists at maturity in mutant, while it
is replaced by A-pattern in parent. Amyloplasts of mutant are modifie
d in contrast to normal, oblong amyloplasts of parent. Modified amylop
lasts show loss of grana, increased granulation and lipid droplets. Ex
tensive lipid complexing with starch polymers in mutant is indicated a
nd possible commercial and nutritional potentials speculated.