THE EFFECT OF VESICAL OUTLET OBSTRUCTION ON THE PROTEIN SECONDARY STRUCTURE OF THE MUCOSA AND SEROSA IN RABBIT BLADDER WALL

The biochemical composition of physiologically moist mucosa and serosa of rabbit bladder before and after bladder outlet obstruction was det ermined by means of FT-IR spectroscopy with the ATR method and second- derivative analysis. A predominantly beta-sheet structure was found in the amide I band for mucosa and serosa before and after obstruction, but the random coil structure increased in both obstructed bladder sam ples. However, the major beta-sheet structure associated with some alp ha-helical structure in the amide II band of mucosa and serosa for non -obstructed bladder changed into a predominantly alpha-helical structu re after bladder obstruction. The obstructed bladder serosa was more p ronounced. The amount of glycoproteins doubled in the obstructed bladd er serosa, but did not change in the bladder mucosa.