The lectin I from Ulex europaeus (UEAI) has a strong affinity for the
H-type 2 human blood group determinant. Single crystals of UEAI have b
een grown in the monoclinic crystal system. Initial crystals were obta
ined after 11 months from a solution of 10 mg/ml protein, 40% 2,4-meth
ylpentanediol and 0.1 N acetate buffer at pH 5.2. The technique of was
hing and reseeding was used to generate large suitable crystals. The s
pace group is C2 with a = 78.84 Angstrom, b = 69.85 Angstrom, c = 120.
62 Angstrom, beta = 108.74 degrees and Z = 4; there is one molecular d
imer per asymmetric unit and the solvent content is estimated to be 58
%. The crystals diffract to at least 2.8 Angstrom d spacings and are s
table in the X-ray beam for more than three days.