CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC CHARACTERIZATION OF ENDO-POLYGALACTURONASE-II FROM ASPERGILLUS-NIGER

Authors
SCHROTER KH ARKEMA A KESTER HCM VISSER J DIJKSTRA BW
Citation
Kh. Schroter et al., CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC CHARACTERIZATION OF ENDO-POLYGALACTURONASE-II FROM ASPERGILLUS-NIGER, Journal of Molecular Biology, 243(2), 1994, pp. 351-352
Citations number
6
Categorie Soggetti
Biology
Journal title
Journal of Molecular Biology → ACNP
ISSN journal
00222836
Volume
243
Issue
2
Year of publication
1994
Pages
351 - 352
Database
ISI
SICI code
0022-2836(1994)243:2<351:CAPCCO>2.0.ZU;2-3
Abstract
The endo-polygalacturonase II from Aspergillus niger has been crystall ized from an ammonium sulfate solution by the hanging drop method. The crystals belong to the monoclinic space group P2(1), with cell dimens ions a = 60.6 Angstrom, b = 152.6 Angstrom, c = 74.0 Angstrom and beta = 91.2 degrees with four molecules per asymmetric unit. The crystals diffract to at least 2.8 Angstrom resolution and are suitable for X-ra y analysis.