A. Kumar et Mn. Gupta, AFFINITY PRECIPITATION OF TRYPSIN WITH SOYBEAN TRYPSIN-INHIBITOR LINKED EUDRAGIT S-100, Journal of biotechnology, 37(2), 1994, pp. 185-189
Soybean trypsin inhibitor linked to Eudragit S-100 was used for the af
finity precipitation of trypsin. Polymer and ligand concentrations use
d in conjugate preparation showed remarkable effect on the trypsin rec
overy. Trypsin precipitation efficiency amounted to 89% and recovery w
as 74%. The final purification of relatively crude commercial trypsin
resulted in 1.85-fold purification. The SDS-PAGE analysis indicated si
gnificant purification. The precipitated enzyme activity was around 96
% and recovered enzyme activity was 83%.