G-PROTEIN AND CAPACITATIVELY REGULATED CA2-LINE( ENTRY PATHWAYS ARE ACTIVATED BY MUSCARINIC RECEPTOR STIMULATION IN A HUMAN SUBMANDIBULAR DUCTAL CELL)

In the human submandibular ductal cell line (HSG) thapsigargin and car bachol stimulated Ca2+ re lease from the internal Ca2+ pool, resulting in the activation of capacitatively regulated Ca2+ entry (CRCE). This entry pathway was permeant to both Ca2+ and Mn2+, blocked by Ni2+ and insensitive to the muscarinic antagonist, atropine. Carbachol also st imulated an increase in cytosolic [Ca2+] in internal Ca2+-pool-deplete d (i.e.thapsigargin-treated) cells which was dependent on the presence of external Ca2+ and blocked by Ni2+, demonstrating that it was due t o Ca2+ entry. However, under the same experimental conditions, carbach ol was unable to stimulate Mn2+ entry. Additionally, this latter carba chol-stimulated Ca2+ entry pathway was blocked by atropine. Pretreatme nt of HSG cells with AlF4-, increased basal rates of Mn2+ entry due to CRCE activation, but at tenuated carbachol-stimulated Ca2+ entry into thapsigargin-treated cells. The data suggest that two distinct divale nt cation entry pathways are activated in muscarinic-receptor-stimulat ed HSG cells; a CRCE mechanism, permeable to both Mn2+ and Ca2+, and a second entry mechanism, permeable only to Ca2+. The latter does not d epend on internal pool depletion, but appears to be regulated via G-pr otein activation.