CHARACTERIZATION OF THE GENE ENCODING MANGANESE PEROXIDASE ISOZYME-3 FROM PHANEROCHAETE-CHRYSOSPORIUM

The gene encoding manganese peroxidase isozyme 3 (MnP3) from the white -rot basidiomycete Phanerochaete chrysosporium was cloned and sequence d, The mnp3 gene encodes a mature protein of 357 amino acids with a 25 amino-acid signal peptide. The amino acids involved in peroxidase fun ction, as well as those forming the Mn-II binding site and those invol ved in disulfide bond formation, are conserved in the MnP3 sequence. T he mnp3 gene has six introns, indicating that the sequenced P. chrysos porium mnp genes can be divided into three subfamilies on the basis of intron-exon structure. The mnp3 gene promoter contains putative metal response elements and heat shock elements which may be involved in th e regulation of mnp gene transcription by Mn, the substrate for the en zyme, and by heat shock. (C) 1997 Elsevier Science B.V.