O. Carrette et al., PURIFICATION AND CHARACTERIZATION OF PIG INTER-ALPHA-INHIBITOR AND ITS CONSTITUTIVE HEAVY-CHAINS, Biochimica et biophysica acta. Protein structure and molecular enzymology, 1338(1), 1997, pp. 21-30
With the view of investigating the metabolism of inter-alpha-inhibitor
, a plasma serine-proteinase inhibitor, in an animal model of inflamma
tory syndrome, we isolated inter-alpha-inhibitor from pig plasma. A hi
gh yield was obtained (140 mg/liter) with a two-step procedure: anion-
exchange chromatography followed by affinity chromatography on heparin
-Sepharose. In contrast to bovine inter-alpha-inhibitor, pig inter-alp
ha-inhibitor was highly similar to human inter-ct-inhibitor: its heavy
chains are homologous to the human H1 and H2 heavy chains, as shown b
y chromatographic and electrophoretic properties, cross-immunoreactivi
ty and N-terminal sequencing. Pig may therefore represent a good anima
l model to study inter-alpha-inhibitor metabolism and elucidate its ph
ysiological role.