An amperometric glucose biosensor based on a new mediator and carbon p
aste acts at 0.08 V vs. an Ag/AgCl electrode in situ and exhibits a li
near response up to 16 mM of glucose. The measurements are performed o
n freshly renovated electrode surfaces, and every sensor can be used f
or 100 measurements. The long-term stable biosensors were obtained usi
ng stabilized glucose oxidase and an array of biosensors. The applicat
ion of a chemometric method permits exploitation of the biosensor duri
ng a period of more than 166 days at 35-degrees-C and relative humidit
y 75%. The relative error of chemometric prediction was less than 5.4%
, and the response changes less than 0.15% per week.