Gr. Fanning et al., ORIGIN OF POSTDEPOLARIZATION HYPERPOLARIZATIONS IN A GREASE-GAP RECORDING PREPARATION, Archives internationales de pharmacodynamie et de therapie, 327(3), 1994, pp. 355-362
In grease-gap recording preparations, depolarizing responses evoked by
agonists are often followed by a hyperpolarization (post-depolarizati
on hyperpolarization). We have investigated the origin of these post-d
epolarization hyperpolarizations in the rat CA1-subiculum slice. They
were evoked by L-glutamate, N-methyl-d-aspartate or ha-amino-3-hydroxy
-5-methyl-isoxazole-4-propionate in approximately 80 % of the slices t
ested. The post-depolarization hyperpolarizations evoked by perfusion
of N-methyl-d-aspartate through the CA1 compartment of the chamber, pe
rsisted when N-methyl-d-aspartate receptors in the subicular compartme
nt were blocked with D-2-amino-5-phosphonopentanoate. Carbachol only b
locked the post-depolarization hyperpolarizations evoked by ha-amino-3
-hydroxy-5-methyl-isoxazole-4-propionate at concentrations which also
blocked the depolarization. The post-depolarization hyperpolarization
was selectively blocked by perfusion with Ca2+-free medium and by admi
nistration of ouabain, and showed a marked sensitivity to temperature.
It is concluded that the post-depolarization hyperpolarizations obser
ved in this preparation are not a consequence of diffusion of the agon
ist through the slice. The evidence is, however, consistent with them
being generated by activation of the electrogenic Na+-K+ pump, althoug
h we cannot exclude an additional contribution from Ca2+ - or voltage-
dependent K+ currents.