Kcs. Liu et al., THE EFFECT OF PLANT-GROWTH REGULATORS ON THE PRODUCTION OF CANTHIN-6-ONE ALKALOIDS BY BRUCEA-JAVANICA CELL-SUSPENSION CULTURES, Phytochemistry, 37(2), 1994, pp. 421-424
Cell suspension cultures of Brucea javanica were grown in media with a
variety of plant growth regulators. Although quassinoid production co
uld not be initiated, high yields of canthin-6-one alkaloids were foun
d. The plant growth regulator regimes used, IAA + KIN, 2,4-D + KIN, NA
A + KIN, NAA + BAP, 2,4-D + BAP and IAA + BAP affected cell growth and
overall alkaloid production. Variations in the yields of the individu
al alkaloids were also observed. The most efficient production of cant
hin-6-one, 11-hydroxycanthin-6-one, 11-methoxycanthin-6-one and 5-meth
oxycanthin-6-one was found in media containing NAA + BAP.