DEVELOPMENTAL ASPECTS OF DETOXIFYING ENZYMES IN FISH (SALMO-IRIDAEUS)

The activities of superoxide dismutase, catalase, glutathione reductas e, glutathione peroxidase, glutathione transferase and glyoxalase I ha ve been studied during the embryologic development of rainbow trout (S almo iridaeus) and in several other trout tissues to investigate the p rotective development metabolism. A gradual increase of superoxide dis mutase, catalase, glutathione reductase, glyoxalase I and glutathione transferase activities was noted throughout embryo development. In all trout tissues investigated glutathione peroxidase was found to be ext remely low compared to catalase activity. The highest activity of supe roxide dismutase, glyoxalase I and glutathione reductase was found in liver followed by kidney. No change in the number of GST subunits was noted with the transition from the embryonic to the adult stages of li fe according to the SDS/PAGE and HPLC analyses performed on the GSH-af finity purified fractions.