DISCRIMINATION OF EPIDEMIC AND SPORADIC ISOLATES OF ACINETOBACTER-BAUMANNII BY REPETITIVE ELEMENT PCR-MEDIATED DNA-FINGERPRINTING

In 1990, there was a significant increase in the number of lower respi ratory tract infections and surgical wound infections in the adult int ensive care units of our tertiary care teaching hospital caused by Aci netobacter baumannii compared with the number in 1989. During the 5-mo nth period from April through August 1990, 84 isolates of A. baumannii were recovered from 50 hospitalized patients. Biotyping, comparison o f antibiograms, plasmid analysis, and DNA polymorphisms of 20 isolates from 20 different patients, determined by the use of repetitive eleme nt PCR,vith primers aimed at repetitive extragenic palindromic sequenc es and enterobacterial repetitive intergenic consensus sequences, were used to investigate this apparent outbreak. Biotyping, antibiograms, plasmid analysis, and enterobacterial repetitive intergenic consensus PCR were not useful epidemiologically. Repetitive element PCR-mediated DNA fingerprinting using repetitive extragenic palindromic primers di scriminated between epidemic and sporadic strains of A. baumannii and demonstrated four discrete clusters which were unique epidemiologicall y.