A LARGE OPEN READING FRAME (ORF1995) IN THE CHLOROPLAST DNA OF CHLAMYDOMONAS-REINHARDTII ENCODES AN ESSENTIAL PROTEIN

Authors
BOUDREAU E TURMEL M GOLDSCHMIDTCLERMONT M ROCHAIX JD SIVAN S MICHAELS A LEU S
Citation
E. Boudreau et al., A LARGE OPEN READING FRAME (ORF1995) IN THE CHLOROPLAST DNA OF CHLAMYDOMONAS-REINHARDTII ENCODES AN ESSENTIAL PROTEIN, MGG. Molecular & general genetics, 253(5), 1997, pp. 649-653
Citations number
15
Categorie Soggetti
Genetics & Heredity",Biology
Journal title
MGG. Molecular & general genetics → ACNP
ISSN journal
00268925
Volume
253
Issue
5
Year of publication
1997
Pages
649 - 653
Database
ISI
SICI code
0026-8925(1997)253:5<649:ALORF(>2.0.ZU;2-B
Abstract
An open reading frame potentially encoding a protein of 1995 amino aci ds (orf1995) has been found in the chloroplast genome of the green alg a Chlamydomonas reinhardtii. Besides having a short hydrophobic N-term inal domain with five putative transmembrane helices, the predicted or f1995 product is highly basic. orf1995 might be a homologue of the ycf 1 gene in land plants, whose function has not yet been determined. Mut ants of C. reinhardtii transformed with a disruption of orf1995 remain heteroplasmic for the wild-type and disrupted alleles of this gene, i ndicating that the orf1995 product is essential for cell survival.