Zl. Herd et Di. Bernstein, ANTIGEN-SPECIFIC STIMULATION OF HISTAMINE-RELEASING FACTORS IN DIISOCYANATE-INDUCED OCCUPATIONAL ASTHMA, American journal of respiratory and critical care medicine, 150(4), 1994, pp. 988-994
Citations number
29
Categorie Soggetti
Emergency Medicine & Critical Care","Respiratory System
Diisocyanate-induced asthma differs from occupational asthma (OA) caus
ed by protein allergens in that specific IgE antibody responses are ra
rely identified. To investigate the immunopathogenesis of diisocyanate
asthma, diisocyanate-exposed workers were evaluated for in vitro prod
uction of antigen-specific mononuclear cell-derived histamine releasin
g factor (HRF). The mean HRF response to diisocyanate-HSA antigens was
significantly greater in patients with OA than in diisocyanate-expose
d asymptomatic subjects (p < 0.05). No association was found between H
RF and diisocyanate-specific antibodies. Analysis of HRF production by
subpopulations of peripheral blood mononuclear cells (PBMC) showed th
at lymphocytes and adherent cells were major sources of both spontaneo
us and antigen-stimulated HRF. The results suggest that antigen-specif
ic HRF produced by PBMCs ate an important biomarker for diisocyanate-i
nduced asthma. This is the first report of hapten-specific stimulation
of PBMCs resulting in HRF production.