ANALYSIS OF GEOMETRICAL PARAMETERS AND ME CHANICAL-PROPERTIES OF ERYTHROCYTES BY FILTRATION THROUGH MEMBRANE NUCLEAR FILTERS .2. EXPERIMENTAL-VERIFICATION OF THE THEORETICAL-MODEL

In the accompanying paper the process of erythrocyte passage through p ores of membrane nuclear filters has been modelled. In order to test t he model we have studied how the filtration rate of human erythrocyte suspension depends on osmoticity of the resuspending medium at various experimental conditions. We have tested and verified the model conclu sion that the zero filtration rate is reached at the critical osmotici ty value which depends only on geometrical parameters of the pore and the cell and not on other properties of the erythrocyte. It has been d emonstrated that the relative filtration rate only weakly depends on t he hydrostatic pressure, in agreement with the model predictions. Ther e is a good correspondence between the model and the experimental data about how the filtration rate is influenced by the viscosity of the i ntracellular content and the mechanical characteristics of the erythro cyte membrane at varions temperatures.