MICRO ENZYMATIC ASSAY COUPLED TO CAPILLARY ELECTROPHORESIS VIA LIQUID-JUNCTION

A system for capillary electrophoresis combined with enzymatic assay h as been evaluated for the two enzymes glucose-6-phosphate dehydrogenas e and 6-phosphogluconate dehydrogenase. Instrumentation included a pos t-column reactor coupled to the separation capillary by a liquid junct ion. A technique for generating a substrate solution flow into the rea ctor by utilizing two high voltage supplies is proposed. This method o ffers a high degree of freedom in optimizing the separation and enzyma tic reaction conditions individually. Possibilities for improving the enzymatic assay sensitivity were also examined.