A CAPILLARY ELECTROPHORESIS ASSAY FOR THE FIBRINOLYTIC AGENT, EMINASE

Authors
BIRRELL HC BRIGHTWELL MD CAMILLERI P
Citation
Hc. Birrell et al., A CAPILLARY ELECTROPHORESIS ASSAY FOR THE FIBRINOLYTIC AGENT, EMINASE, Chromatographia, 39(5-6), 1994, pp. 325-328
Citations number
5
Categorie Soggetti
Chemistry Analytical
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
39
Issue
5-6
Year of publication
1994
Pages
325 - 328
Database
ISI
SICI code
0009-5893(1994)39:5-6<325:ACEAFT>2.0.ZU;2-0
Abstract
Capillary eIectrophoresis (CE) has been used to develop a method that allows analysis for all the main components of Eminase. This technique has also resolved the two glycosylated forms of lys-plasminogen. Quan titative CE analysis of concentration levels of this enzyme complex ha ve been directly compared to those obtained from a bio-assay. A linear relation between the two methods has been demonstrated. Moreover, the CE method is highly reproducible, combines precision with speed, and allows quantitative analysis of the other protein components (streptok inase and human serum albumin) in Eminase.