VALIDATION OF A STRATEGIC APPROACH TO HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD SELECTION

A recently reported chromatographic method selection strategy has been validated using fifteen drug formulations selected randomly from the Belgium Compendium, 1992. On the basis of the hydrophobic and the acid ic - basic properties of the sample components, reversed-phase was rec ommended as the first choice mode for all formulations. For two multic omponent formulations the range of analyte polarity dictated the need for gradient elution. The commercial software DryLab G/plus(R) was use d for selection of optimum gradient conditions. The results obtained b y both isocratic and gradient chromatography are discussed, as is the usefulness of a tailing suppressor in both modes.