EFFECTIVENESS OF TALC AS ADSORBENT FOR PURIFICATION AND IMMOBILIZATION OF PLANT LIPOXYGENASES

Authors
BATTU S RABINOVITCHCHABLE H BENEYTOUT JL
Citation
S. Battu et al., EFFECTIVENESS OF TALC AS ADSORBENT FOR PURIFICATION AND IMMOBILIZATION OF PLANT LIPOXYGENASES, Journal of agricultural and food chemistry, 42(10), 1994, pp. 2115-2119
Citations number
21
Categorie Soggetti
Food Science & Tenology",Agriculture,"Chemistry Applied
Journal title
Journal of agricultural and food chemistry → ACNP
ISSN journal
00218561
Volume
42
Issue
10
Year of publication
1994
Pages
2115 - 2119
Database
ISI
SICI code
0021-8561(1994)42:10<2115:EOTAAF>2.0.ZU;2-R
Abstract
The present study describes the immobilization of lipoxygenase from So lanum tuberosum tuber and Lupinus albus, Cicer arietinum, and Pisum sa tivum seeds on a magnesium silicate based support, talc. In all cases, an increase in specific activity of many hundreds of units per millig ram was obtained and the use of free or immobilized enzymes was quite similar. The adsorption of plant lipoxygenases on talc was a direct, r apid, and inexpensive method to purify and stabilize these enzymes.