INTERACTIONS OF PLASMA-PROTEINS WITH A NOVEL POLYSACCHARIDE SURFACTANT PHYSISORBED TO POLYETHYLENE

A polysaccharide surfactant, dextran-[1,6 bis(2-hydroxypropyl-1-amine) hexane]-dextran, (D-H-D) was prepared by reacting dextran (M(w) = 8200 ) with epichlorohydrin followed by reaction with 1,6-hexanediamine. Th e D-H-D polymer product was characterized by gel permeation chromatogr aphy (GPC), and C-13-nuclear magnetic resonance spectroscopy (C-13-NMR ). D-H-D was physisorbed on polyethylene (PE) from aqueous solution, a nd the adhesion stability and resistance to protein adsorption was exa mined under static and dynamic flow conditions, using a modified rotat ing disk system. Modified surfaces were characterized by attenuated to tal reflectance Fourier transformed infrared spectroscopy (ATR-FTIR), electron spectroscopy for chemical analysis (ESCA) and by water contac t angles. Under applied shear stresses of up to 73 dyn cm(-2), the adh esion of D-H-D on PE was sufficient to inhibit desorption by water (>9 0% D-H-D on PE was retained) and 5% SDS surfactant solution (approxima tely 83% D-H-D retained), as determined by ATR-FTIR. Under similar she ar stress conditions, albumin adsorption on D-H-D modified PE was redu ced by over 90%, and protein adsorption from fresh human plasma was re duced by approximately 70% compared with unmodified PE. The results ar e discussed in terms of interfacial forces, and the suitability of thi s approach for studying protein-surface interactions and for developin g a novel class of protein-resistant biomaterials.