BIOTRANSFORMATION OF THE NATURALLY-OCCURRING LIGNAN (-)-ARCTIGENIN INMAMMALIAN-CELL LINES GENETICALLY-ENGINEERED FOR EXPRESSION OF SINGLE CYTOCHROME-P450 ISOFORMS

Authors
KASPER R GANSSER D DOEHMER J
Citation
R. Kasper et al., BIOTRANSFORMATION OF THE NATURALLY-OCCURRING LIGNAN (-)-ARCTIGENIN INMAMMALIAN-CELL LINES GENETICALLY-ENGINEERED FOR EXPRESSION OF SINGLE CYTOCHROME-P450 ISOFORMS, Planta medica, 60(5), 1994, pp. 441-444
Citations number
23
Categorie Soggetti
Pharmacology & Pharmacy","Plant Sciences
Journal title
Planta medica → ACNP
ISSN journal
00320943
Volume
60
Issue
5
Year of publication
1994
Pages
441 - 444
Database
ISI
SICI code
0032-0943(1994)60:5<441:BOTNL(>2.0.ZU;2-3
Abstract
V79 Chinese hamster cells genetically engineered for stable expression of single forms of rat cytochromes P450 1A1, P450 1A2, and P450 2B1, and human P450 1A2 were used to analyse the metabolism of (-)-arctigen in. Concentrations of metabolites formed in the medium were determined by TLC, HPLC, GC, and GC-MS. Only in the cell line expressing rat P45 0 2B1 was (-)-artigenin metabolized to a significant extent. The ligna n was converted by O-demethylation to (-)-3'-O-demethylarctigenin.