CONSTRUCTION AND CHARACTERISTICS OF ARRAY ED LIBRARY OF TRANSCRIBED HUMAN CHROMOSOME-19 SEQUENCES FROM THE HUMAN-HAMSTER HYBRID CELL-LINE

Authors
OBRADOVITCH D BORODIN AM KOPANTZEV EP WAGNER LL VOLIK SV ERMOLAEVA OD LEBEDEV YB MONASTYRSKAYA GS SVERDLOV ED
Citation
D. Obradovitch et al., CONSTRUCTION AND CHARACTERISTICS OF ARRAY ED LIBRARY OF TRANSCRIBED HUMAN CHROMOSOME-19 SEQUENCES FROM THE HUMAN-HAMSTER HYBRID CELL-LINE, Bioorganiceskaa himia, 20(8-9), 1994, pp. 919-931
Citations number
33
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
20
Issue
8-9
Year of publication
1994
Pages
919 - 931
Database
ISI
SICI code
0132-3423(1994)20:8-9<919:CACOAE>2.0.ZU;2-M
Abstract
A new technique based on Alu-PCR amplification of hn-RNA is described for the extraction of human-specific transcribed sequences from a hybr id cell line. Arrayed library of hn-cDNA was constructed and character ized by sequencing about 80 individual clones. A high enrichment by hu man-specific sequences (about 95%) was demonstrated.