Sg. Vorsanova et al., RAPID IDENTIFICATION OF MARKER CHROMOSOMES BY IN-SITU HYBRIDIZATION UNDER DIFFERENT STRINGENCY CONDITIONS, Analytical cellular pathology, 7(3), 1994, pp. 251-258
Six metacentric non-satellite chromosome markers and 4 satellite marke
rs of unknown origin were discovered by routine cytogenetic analysis.
These markers were then investigated by isotopic and nonisotopic (FISH
) in situ hybridization. In order to determine the origin of small mar
ker chromosomes, a special protocol involving sequential application o
f defined alphoid and 'classical' satellite DNA probes with chromosome
specificity was used. In situ hybridization under low stringency cond
itions has been performed with DNA probes specific for 4 groups of chr
omosomes. After preliminary analysis and determination of the possible
origin of these marker chromosomes, DNA probes with high chromosome s
pecificity were used under high stringency conditions. Marker chromoso
mes were found to be derivatives of chromosomes 7, 9 (3 cases), 13, 14
or 22, 21 (2 cases), X and Y.