SINGLE-PASS SEQUENCING OF A UNIDIRECTIONAL HUMAN FETAL HEART CDNA LIBRARY TO DISCOVER NOVEL GENES OF THE CARDIOVASCULAR-SYSTEM

Authors
HWANG DM HWANG WS LIEW CC
Citation
Dm. Hwang et al., SINGLE-PASS SEQUENCING OF A UNIDIRECTIONAL HUMAN FETAL HEART CDNA LIBRARY TO DISCOVER NOVEL GENES OF THE CARDIOVASCULAR-SYSTEM, Journal of Molecular and Cellular Cardiology, 26(10), 1994, pp. 1329-1333
Citations number
18
Categorie Soggetti
Cardiac & Cardiovascular System
Journal title
Journal of Molecular and Cellular Cardiology → ACNP
ISSN journal
00222828
Volume
26
Issue
10
Year of publication
1994
Pages
1329 - 1333
Database
ISI
SICI code
0022-2828(1994)26:10<1329:SSOAUH>2.0.ZU;2-U
Abstract
A human fetal heart cDNA library was constructed in the lambda gt22A e xpression vector. Polymerase chain reaction (PCR) was used to amplify the cDNA inserts. PCR products were purified and used in cycle sequenc ing reactions in the presence of a fluorescein-conjugated primer and e lectrophoresed on a Pharmacia A.L.F. Sequencer. Partial cDNA sequences , or expressed sequence tags (ESTs) were searched against the Genbank and EMBL databases to identify novel genes expressed in the human card iovascular system.