ADSORPTION OF ANTIBODIES TO A LANGMUIR LAYER OF OCTADECYLAMINE AND THE INTERACTION WITH ANTIGEN

The adsorption of antibodies against C-reactive protein onto a monolay er of octadecylamine has been studied at the air/water interface by fo llowing the change in area of the monolayer at different surface press ures. Binding of antibodies was dependent both on the packing of the f ilm and on the antibody concentration in the aqueous subphase. An equi librium was reached above an antibody concentration of 2 mg/dm(3). The monolayer was stabilized by the antibodies. The mixed monolayer could be transferred onto a quartz crystal microbalance. However, the high nonspecific binding of bovine serum albumin makes it difficult to dete rmine the interaction of the antigen with the octadecylamine/antibody layer. (C) 1994 Academic Press, Inc.