SENSITIVE FLUORESCENCE DETECTION IN CAPILLARY ELECTROPHORESIS USING LASER-DIODES AND MULTIPLEX DYES

Authors
BACHTELER G DREXHAGE KH ARDENJACOB J HAN KT KOLLNER M MULLER R SAUER M SEEGER S WOLFRUM J
Citation
G. Bachteler et al., SENSITIVE FLUORESCENCE DETECTION IN CAPILLARY ELECTROPHORESIS USING LASER-DIODES AND MULTIPLEX DYES, Journal of luminescence, 62(3-4), 1994, pp. 101-108
Citations number
24
Categorie Soggetti
Optics
Journal title
Journal of luminescence → ACNP
ISSN journal
00222313
Volume
62
Issue
3-4
Year of publication
1994
Pages
101 - 108
Database
ISI
SICI code
0022-2313(1994)62:3-4<101:SFDICE>2.0.ZU;2-W
Abstract
A detector for laser induced fluorescence based on laser diode technol ogy was designed and sensitivity measurements with rhodamine 800 were performed. The detection limit is around 100 molecules in the detectio n volume. Using the laser diode in combination with a commercial capil lary electrophoresis system, about 1200 molecules were detectable. Usi ng time-resolved fluorescence measurements multiplex dyes were recogni zed with a pulsed laser diode and a pattern recognition technique coll ecting only a few hundred photons.