SYNTHESIS AND PRELIMINARY CHEMILUMINESCENCE AND BIOLUMINESCENCE STUDIES OF A NOVEL PHOTOLABILE COELENTERAZINE ANALOG WITH A TRIFLUOROMETHYLDIAZIRINE GROUP

Authors
CHEN FQ HIRANO T HASHIZUME Y OHMIYA Y OHASHI M
Citation
Fq. Chen et al., SYNTHESIS AND PRELIMINARY CHEMILUMINESCENCE AND BIOLUMINESCENCE STUDIES OF A NOVEL PHOTOLABILE COELENTERAZINE ANALOG WITH A TRIFLUOROMETHYLDIAZIRINE GROUP, Journal of the Chemical Society, Chemical Communications, (20), 1994, pp. 2405-2406
Citations number
23
Categorie Soggetti
Chemistry
Journal title
Journal of the Chemical Society, Chemical Communications → ACNP
ISSN journal
00224936
Issue
20
Year of publication
1994
Pages
2405 - 2406
Database
ISI
SICI code
0022-4936(1994):20<2405:SAPCAB>2.0.ZU;2-D
Abstract
A novel photolabile analogue of coelenterazine with a trifluoromethyl diazirine group is successfully synthesized for photoaffinity labellin g of the active site of aequorin; preliminary studies on its chemi- an d bio-luminescence demonstrate that the photolabile analogue shows the same luminescence properties and kinetics as those of natural coelent erazine and thus, it is deduced that both compounds occupy the same ac tive site; of aequorin.