NOVEL ANTIBIOTICS, AMYTHIAMICINS .3. STRUCTURE ELUCIDATIONS OF AMYTHIAMICIN-A, AMYTHIAMICIN-B AND AMYTHIMICIN-C

The structures of novel antimicrobial antibiotics, amythiamicins A, B and C, were elucidated by chemical degradations and NMR spectral analy ses. The main frame from C-1 to C-41 of these antibiotics was the same as that of amythiamicin D. Amino acid autoanalyses of amythiamicins A , B and C showed that these have another one mole of serine and prolin e in comparison with amythiamicin D. Stereochemistries of both amino a cids were determined to be L by chiral HPLC. These seryl-prolyl residu es in amythiamicins A, B and C are attached at G41 through an oxazolin e ring, amide and ester bond, respectively.