Kcm. Raja et Sv. Ramakrishna, IMPROVED REACTION CONDITIONS FOR PREPARATION OF BETA-CYCLODEXTRIN (BETA-CD) FROM CASSAVA (MANIHOT-ESCULENTA CRANTZ) STARCH, Starke, 46(10), 1994, pp. 402-403
Experimental conditions for bioconversion of starch to get higher yiel
d of beta-cyclodextrin using cyclodextrin glycosyl transferase (CGT-as
e) (EC 2.4.1.19) were worked out. A 5% initial starch concentration ga
ve beta-CD yield of 20-28% that could be separated as beta-CD bromoben
zene complex from the reaction mixture. Addition of bromobenzene durin
g the second phase of the reaction, i.e. CD formation phase, was found
beneficial.