A COMPARISON BETWEEN POTENCIES OF EXTERNAL CALCIUM, STRONTIUM AND BARIUM TO SUPPORT GABAERGIC SYNAPTIC TRANSMISSION IN RAT CULTURED HIPPOCAMPAL-NEURONS

Relative potencies of external Ca2+, Sr2+ and Ba2+ to trigger GABAergi c synaptic transmission were evaluated by applying the patch-clamp tec hnique to both presynaptic and postsynaptic hippocampal neurons prepar ed from neonatal rats. Action potentials were evoked by application of voltage pulses to presynaptic neurons, and GABAergic synaptic current s were recorded in voltage-clamped postsynaptic neurons. No stimuli we re delivered during replacement with test solutions and only five puls es were applied to the presynaptic neuron in each test solution. Durin g the five-pulse application, the amplitude of synaptic currents was c onstant in Ca2+-containing solutions, but decreased successively in Ba 2+- and Sr2+-containing solutions without Ca2+. Thus, the amplitude of synaptic currents induced by the first pulse in each ionic condition was used to evaluate the potency of divalent cations. The lowest exter nal concentration required to trigger the transmission was 0.3 mM for Ca2+, 1 mM for Sr2+ and 2 mM for Ba2+, and the concentration required to achieve the same effect as with 2 mM Ca2+ was 6 mM for Sr2+ and 10 mM for Ba2+. These results strongly suggest that Ba2+ as well as Sr2can be substituted for Ca2+ in GABAergic synaptic transmission and the order of potency is Ca2+ > Sr2+ > Ba2+.