Rs. Agrawal et al., FLOW CYTOMETRIC QUANTITATION OF C-MYC AND P53 PROTEINS IN BOVINE PAPILLOMAVIRUS TYPE 1-TRANSFORMED PRIMARY MOUSE FIBROBLASTS, Cytometry, 17(3), 1994, pp. 237-245
Citations number
38
Categorie Soggetti
Cytology & Histology","Biochemical Research Methods
Bovine papillomavirus type 1 (BPV-1)-transformed mouse fibroblast cell
lines were analyzed via flow cytometry (FCM) for expression of p53 an
d c-myc proteins along with their DNA content. In comparison to the no
ntransformed control cell line, significantly elevated levels of both
the p53 and the c-myc protein were present in some but not all of the
transformed cell lines. Quantitation of p53 and c-myc proteins in cell
lines containing BPV-1 DNA revealed that the tumorigenic cell lines e
xpressed higher levels of both the p53 (P = 0.0034; Mann-Whitney U tes
t) and the c-myc protein (P = 0.0039; Mann-Whitney U test) as compared
to the nontumorigenic cell lines. On average, at least 9,000-10,000 p
53 or c-myc protein molecules per cell were detected in the transforme
d tumorigenic cell lines. These results show that quantitative FCM can
be reliably used to detect very low levels (3,000 molecules per cell)
of specific protein, and FCM is a useful tool to study the virus-indu
ced changes in the levels of nuclear proteins within a cell population
and in tumorigenesis. (C) 1994 Wiley-Liss, Inc.