CHEMICAL-ANALYSIS AND BIOLOGICAL TESTING OF A POLAR FRACTION OF AMBIENT AIR, DIESEL-ENGINE, AND GASOLINE-ENGINE PARTICULATE EXTRACTS

Authors
STRANDELL M ZAKRISSON S ALSBERG T WESTERHOLM R WINQUIST L RANNUG U
Citation
M. Strandell et al., CHEMICAL-ANALYSIS AND BIOLOGICAL TESTING OF A POLAR FRACTION OF AMBIENT AIR, DIESEL-ENGINE, AND GASOLINE-ENGINE PARTICULATE EXTRACTS, Environmental health perspectives, 102, 1994, pp. 85-92
Citations number
20
Categorie Soggetti
Public, Environmental & Occupation Heath","Environmental Sciences
Journal title
Environmental health perspectives → ACNP
ISSN journal
00916765
Volume
102
Year of publication
1994
Supplement
4
Pages
85 - 92
Database
ISI
SICI code
0091-6765(1994)102:<85:CABTOA>2.0.ZU;2-G
Abstract
Extracts of gasoline and diesel vehicle exhaust and ambient air partic les were fractionated into five fractions according to polarity on a s ilica gel column. Two medium polar fractions showing high genotoxic ac tivity in the Ames test were further subfractionated, using normal-pha se high-performance liquid chromatography. Chemical analyses were perf ormed by means of gas chromatography combined with mass spectrometry a nd flame ionization and detection. The crude extracts, fractions, and subfractions were assayed with the Ames test, with and without S9, and the most abundant compounds in the subfractions are reported.