HUMAN-CHROMOSOME-19P - A FLUORESCENCE IN-SITU HYBRIDIZATION MAP WITH GENOMIC DISTANCE ESTIMATES FOR 79 INTERVALS SPANNING 20-MB

A physical map of human chromosome 19p has been constructed by fluores cence in situ hybridization of cosmids to metaphase chromosomes and sp erm pronuclear interphases. The map spans similar to 20 Mb and was gen erated with 141 multiple, partially overlapping estimates of genomic d istances for 79 intervals separating 80 sequentially ordered cosmid re ference points. The average distance separating pairs of cosmids was 2 50 kb, with a range from 50 to 700 kb; 75% of the intervals were estim ated to be less than or equal to 300 kb and only 8 intervals were betw een 500 and 700 kb. Cosmids positive for 33 genes or gene families and 5 polymorphic markers were included among the mapped elements. The fl uorescence in situ hybridization map will be useful for furthering the integration of the physical and genetic maps of 19p and for placing n ewly identified markers within a few hundred kb of their neighbors. (C ) 1994 Academic Press, Inc.