REGIONAL LOCALIZATION OF HUMAN-CHROMOSOME-15 LOCI

One hundred forty-nine chromosome 15 loci were mapped by PCR with resp ect to chromosome breakpoints in three somatic cell hybrids retaining total or part of chromosome 15 and to a 10-Mb YAC contig. This chromos ome was subdivided into 5 regions, yielding an average resolution of m ore than 1 sequence tagged site per megabase. The mapped loci included 18 genes, 60 cDNA-derived sequence tagged sites, and 69 microsatellit es. In addition, the amount of chromosome 15 retained in line A15.1 ha s been defined. This work represents the first attempt at an integrati on of the human physical, expression, and genetic maps of chromosome 1 5. (C) 1994 Academic Press, Inc.