A HIGH-RESOLUTION GENETIC-MAP OF MOUSE CHROMOSOME-5 ENCOMPASSING THE REELER (RL) LOCUS

Using interspecific crosses between BALB/c and Mus spretus (SEG) mice, the murine reeler (rl) gene was mapped to the proximal region of chro mosome 5 between the hepatocyte growth factor gene (Hgf) and the D5Mit 66 microsatellite. The following order was defined: (centromere)-Cch12 a/Hgf-D5Mit1-D5Nam1/D5Nam2- rl/D51Mit61-D5Mit72-Xmv45-Htr5a-Peplb D5Na m3-D5Mit66. Estimated distances between reeler and the nearest flankin g markers D5Nam1 and D5Mit72 are 1.5 and 1.0 cM, respectively (95% con fidence level), suggesting that the region could be physically mapped using a manageable number of YAC clones. (C) 1994 Academic Press, Inc.