ISOLATION AND IDENTIFICATION OF AN EIKELBOOM TYPE-1863 STRAIN AS ACINETOBACTER-JOHNSONII

Type 1863 strain RT2 was isolated by micromanipulation techniques from an activated sludge sewage treatment plant in Rome. The isolate is a coccobacillus which grows in chains. It is a strict aerobe that is oxi dase negative and catalase positive, exhibits chemoorganotrophic metab olism and grows on acetate as the sole carbon source but does not grow on carbohydrates. Strain RT2 is a member of the genus Acinetobacter b y the transformation assay. The 16S rDNA sequence of RT2 was determine d and used to phylogenetically place the strain in the gamma subclass of the proteobacteria as Acinetobacter johnsonii. (C) 1997 Elsevier Sc ience Ltd.