PLANT-REGENERATION FROM HYPOCOTYL PROTOPLASTS OF WINTER OILSEED RAPE (BRASSICA-NAPUS L)

Authors
ORCZYK W NADOLSKAORCZYK A
Citation
W. Orczyk et A. Nadolskaorczyk, PLANT-REGENERATION FROM HYPOCOTYL PROTOPLASTS OF WINTER OILSEED RAPE (BRASSICA-NAPUS L), Acta Societatis Botanicorum Poloniae, 63(2), 1994, pp. 147-151
Citations number
NO
Categorie Soggetti
Plant Sciences
Journal title
Acta Societatis Botanicorum Poloniae → ACNP
ISSN journal
00016977
Volume
63
Issue
2
Year of publication
1994
Pages
147 - 151
Database
ISI
SICI code
0001-6977(1994)63:2<147:PFHPOW>2.0.ZU;2-3
Abstract
Protoplasts were isolated from hypocotyls of six breeding lines and tw o cultivars of winter oilseed rape (B. napus L.). Under presented cult ure conditions almost all of the protoplasts regenerated cell walls. D ivision frequency depended on the genotype and was from 50% to 64%. Sh oot regeneration (also depended on the genotype) was induced with the frequency of 3.6% (for cv Bolko) on the medium containing IAA (0.1 mg. dm-3), zeatin (0.5 mg.dm-3) and BAP (0.5 mg.dm-3). All shoots were roo ted on MS basal medium supplemented with sucrose 30 g.dm-3.