CYSTEAMINE OXIDATION BY LENTIL SEEDLING AMINE OXIDASE

Authors
MEDDA R PADIGLIA A LORRAI A DEMARCO C FLORIS G
Citation
R. Medda et al., CYSTEAMINE OXIDATION BY LENTIL SEEDLING AMINE OXIDASE, Biochemistry and molecular biology international, 41(2), 1997, pp. 395-405
Citations number
22
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
41
Issue
2
Year of publication
1997
Pages
395 - 405
Database
ISI
SICI code
1039-9712(1997)41:2<395:COBLSA>2.0.ZU;2-4
Abstract
Cysteamine is oxidatively deaminated by lentil amine oxidase. It shows saturation kinetic K-m = 9 x 10(-4) M like other substrates, but the aldehyde produced leads to loss of enzyme activity, which is restored by dialysis. When putrescine is the substrate of the amine oxidase cys teamine behaves like a competitive inhibitor, and shows K-i = 5 x 10(- 5) M. The possible involvement of the oxidation of cysteamine and the inhibitory effects of thioacetaldehyde in the cystamine oxidation by a mine oxidase is discussed.