CHARACTERIZATION OF A CYCLIC COMPOUND FORMED AFTER SPERMINE OXIDATIONBY LENTIL AMINE OXIDASE

Spermine is a substrate of lentil seedling amine oxidase and is oxidiz ed at terminal amino groups to a dialdehyde: 2 mol of hydrogen peroxid e and two mol of ammonia per mol of spermine are formed. In the presen ce of hight amounts of spermine, the aldehydic groups formed upon oxid ation of spermine by the enzyme, may react with primary amino groups o f free spermine leading to the formation of aromatic pyrimidinic ring after beta-elimination at secondary amino groups.