EFFICIENT GENE-TRANSFER IN SYNECHOCOCCUS SP STRAINS PCC-7942 AND PCC-6301 BY INTERSPECIES CONJUGATION AND CHROMOSOMAL RECOMBINATION

Authors
TSINOREMAS NF KUTACH AK STRAYER CA GOLDEN SS
Citation
Nf. Tsinoremas et al., EFFICIENT GENE-TRANSFER IN SYNECHOCOCCUS SP STRAINS PCC-7942 AND PCC-6301 BY INTERSPECIES CONJUGATION AND CHROMOSOMAL RECOMBINATION, Journal of bacteriology, 176(21), 1994, pp. 6764-6768
Citations number
20
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
176
Issue
21
Year of publication
1994
Pages
6764 - 6768
Database
ISI
SICI code
0021-9193(1994)176:21<6764:EGISSS>2.0.ZU;2-#
Abstract
We developed a versatile, efficient genetic transfer method for Synech ococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transfo rmation efficiencies by orders of magnitude. As a test case, we comple mented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.