CHROMOSOMAL LOCALIZATION OF THE PARATHYROID-HORMONE PARATHYROID HORMONE-RELATED PROTEIN-RECEPTOR GENE TO HUMAN-CHROMOSOME 3P21.1-P24.2

The human PTH/PTH-related peptide (PTH/PTHrP) receptor could be involv ed in hereditary disorders of PTH or PTHrP action. Knowledge of the ge ne's chromosomal location would allow studies linking it to specific d isease traits. Therefore, we mapped the human PTH/PTHrP receptor gene by polymerase chain reaction of human/rodent somatic cell hybrid panel s using oligonucleotide primers designed to amplify a portion of the g ene from genomic DNA. The PTH/PTHrP gene was unambiguously assigned to the short arm of human chromosome 3, in the region designated 3p21.1- p24.2. Analysis of a second chromosome 3-specific mapping panel sugges ts that the gene is located near the 3p21.2-p21.3 boundary. The availa bility of highly polymorphic markers located in this region will permi t exploration of the PTH/PTHrP receptor locus in genetic linkage searc hes for the causes of bone, calcium, and other potential disorders.