ADSORPTION OF HUMAN RECOMBINANT ERYTHROPOIETIN ON DIALYSIS MEMBRANES IN-VITRO

The adsorptive characteristics of 5 dialysis membranes for recombinant human erythropoietin (EPO) were studied in vitro in a closed circuit system. For 120 min, EPO added with bovine serum was significantly ads orbed by polymethylmetacrylate (PMMA) and polyacrylonitrile (PAN) memb ranes but not by Cuprophan, ethylene vinyl alcohol (EVAL), or polysulf one (PS) membranes. In addition the EPO adsorptive rate, as well as th e of beta2-microglobulin (beta2-MG), was greater with a PMMA membrane than with a PAN membrane. EPO was not detected in the ultrafiltrate at 15 min with 5 membranes. These results indicate that EPO was eliminat ed by membrane adsorption only with some dialysis membranes.