SPECIFIC HUMAN IGE RESPONSE AFTER IMMUNIZATION WITH BIRCH POLLEN IN SCID MICE TRANSPLANTED WITH HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS - A MODEL FOR HUMAN ALLERGY

C.B-17 scid mice were transplanted ip with human peripheral blood mono nuclear cells. Some transplanted mice were immunized ip with birch pol len. Levels of total and birch pollen-specific human IgE were measured in serum. Low levels of total human IgE were detected after 9 days. 2 0 days after transplantation 12 out of 12 mice transplanted with cells from a birch pollen allergic donor showed higher but variable levels of total human IgE, and a low level of birch pollen specific IgE was d etected in one mouse that had not been immunized. Immunization with bi rch pollen did not influence the level of total human IgE. In a differ ent experiment, mice transplanted with cells from a donor with a high level of total IgE, but without birch pollen allergy, developed high l evels of total IgE 49 days after transplantation, whereas no birch pol len specific IgE was found (two immunized, two non-immunized mice). In contrast, two of four mice given cells from two donors with birch pol len allergy, but with lower levels of total IgE, produced birch pollen -specific IgE after immunization. Thus, measurable levels of total hum an IgE were spontaneously produced in most scid mice transplanted with human peripheral blood mononuclear cells. Detectable levels of birch pollen specific human IgE appeared to be produced in occasional non-im munized scid mice transplanted with cells from allergic donors. In mic e given cells from some allergic donors, half the mice produced human birch pollen specific IgE after immunization.