RNA-TRANSCRIPTION IN AXOTOMIZED DORSAL-ROOT GANGLION NEURONS

Levels of RNA transcription were examined in L5 sensory ganglion neuro ns after unilateral crush injuries of the sciatic nerve using an in si tu autoradiographic technique. Neuronal transcription increased in a b iphasic pattern on the lesioned side within the first two weeks after injury. The timing and pattern of increases in transcription correspon ded to previously reported increases RNA synthesis. The data demonstra te a dynamic regulation of RNA transcription in regenerating neuronal populations. This technique may prove to be useful in the study of fac tors controlling transcriptional activity in injured neurons.