Wh. Yap et al., EXPRESSION OF MOSQUITOCIDAL TOXIN GENES IN A GAS-VACUOLATED STRAIN OFANCYLOBACTER-AQUATICUS, Applied and environmental microbiology, 60(11), 1994, pp. 4199-4202
A series of plasmids bearing the binary toxin genes of Bacillus sphaer
icus 2297 or 2317.3, the 100-kDa toxin gene of B. sphaericus SSII-1, o
r the 130-kDa (cryIVB) toxin gene ofBacillus thuringiensis subsp. isra
elensis were constructed and introduced into Ancylobacter aquaticus by
electroporation. The transformed A. aquaticus cells exhibited signifi
cant toxicity towards mosquito larvae, demonstrating a potential use o
f recombinant A. aquaticus for biological control of mosquitoes.