SITES OF GLYCATION OF BETA-B-2-CRYSTALLIN BY GLUCOSE AND FRUCTOSE

We determined the sites of glycation of bovine beta B-2-crystallin by glucose and fructose. After incubation with glucose or fructose. glyca ted tryptic peptides were purified by affinity chromatography/reverse- phase HPLC and identified by electrospray ionization mass spectrometry (ESIMS). The results gave evidence of glycation at lysine 10, 75, 100 , 107, 120, 139, 167 and 171 by bath glucose and fructose, while glyca ted lysine 119 and 47 or 67 were detected only after fructosylation. W e conclude that glucose and fructose have similar glycation specificit y. (C) 1996 Academic Press, Inc.