Br. Yacyshyn et al., CROHNS-DISEASE, ULCERATIVE-COLITIS, AND NORMAL INTESTINAL LYMPHOCYTESEXPRESS INTEGRINS IN DISSIMILAR PATTERNS, Gastroenterology, 107(5), 1994, pp. 1364-1371
Background/Aims: The integrin family of adhesion molecules on intestin
al lamina propria mononuclear cells (LPMNC) was studied using fluoresc
ence-activated cell cytometry. These molecules are implicated in extra
vascular cell migration and are important regulators of disease. Metho
ds: Using fluorescence-activated cell cytometry, B- and T-cell subsets
in the intestines of 10 normal patients, 11 patients with Crohn's dis
ease, and 8 patients with ulcerative colitis were stained with monoclo
nal antibodies to a panel of integrins. Results: Expression of alpha i
ntegrins on CD3(+) T cells and CD19(+) B cells was different in normal
and inflammatory bowel disease LPMNC. Ulcerative colitis T cells expr
essed less beta 1 and alpha 4 and significantly more alpha 2 and alpha
6. There was a difference in alpha 4 and beta 1 expression between LP
MNC B cells from Crohn's disease and normal intestines. Sixteen percen
t of CD19(+) LPMNC B cells from Crohn's and 19% of ulcerative colitis
LPMNC expressed alpha 2. Crohn's and ulcerative colitis CD19(+) LPMNC
B cells expressed more alpha 5 integrin than normal specimens. CD3(+)
T cells and CD19(+) B cells expressed alpha 6 only in ulcerative colit
is. Ulcerative colitis and Crohn's disease CD19(+) LPMNC expressed les
s alpha 4, consistent with their reciprocal increases of alpha 5 and a
lpha 2. A difference in beta 7 (Peyer's patch specific) antigen was ob
served between inflammatory bowel disease and normal LPMNC for both CD
3(+) and CD19(+) LPMNC. Conclusions: These findings identify the diffe
rences of lymphocyte homing capability in inflammatory bowel disease a
nd normal intestine.