USE OF HGCL2 TO INVESTIGATE DYNAMIC PHENOMENA IN YEAST CYTOPLASM

Authors
THEOBALD U MAILINGER W RIZZI M
Citation
U. Theobald et al., USE OF HGCL2 TO INVESTIGATE DYNAMIC PHENOMENA IN YEAST CYTOPLASM, Biotechnology techniques, 8(10), 1994, pp. 723-728
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
8
Issue
10
Year of publication
1994
Pages
723 - 728
Database
ISI
SICI code
0951-208X(1994)8:10<723:UOHTID>2.0.ZU;2-H
Abstract
To investigate dynamic phenomena in yeast cytoplasm a method was devel oped that guarantees immediate inactivation of cell activity. Differen tial extraction procedure gains porosity of the outer cell membrane ex clusively. Application of the method during transition from glucose Li mitation to glucose excess in Saccharomyces cerevisiae illustrates the dynamic behaviour of yeast metabolism and emphasized the necessity of intracellular compartmentation.