STABLE EXPRESSION OF THE CDNA-ENCODING THE FELINE CD8-ALPHA GENE

Authors
PECORARO MR KAWAGUCHI Y OKITA M INOSHIMA Y TOHYA Y KAI C MIKAMI T
Citation
Mr. Pecoraro et al., STABLE EXPRESSION OF THE CDNA-ENCODING THE FELINE CD8-ALPHA GENE, Journal of veterinary medical science, 56(5), 1994, pp. 1001-1003
Citations number
25
Categorie Soggetti
Veterinary Sciences
Journal title
Journal of veterinary medical science → ACNP
ISSN journal
09167250
Volume
56
Issue
5
Year of publication
1994
Pages
1001 - 1003
Database
ISI
SICI code
0916-7250(1994)56:5<1001:SEOTCT>2.0.ZU;2-W
Abstract
The stable expression of the alpha chain of the feline cytotoxic T cel l differentiation antigen (fCD8alpha) on Crandell feline kidney cells (CRFK) was carried out by using an expression vector which contains th e Rous sarcoma virus long terminal repeat and a neo resistant gene. Af ter three rounds of cloning under G418 selection for over two months, the expression of the feline polypeptide was detected by human monoclo nal antibody OKT8.